FIG. 6.

The P. profundum SS9 PBPRA1039 gene complements the P. profundum FL28 mutant at 0.1 MPa but is lethal at 28 MPa. Cultures of the strains shown were grown in marine broth and streptomycin until early stationary phase, and then 10-μl aliquots were spotted onto low-gelling-temperature marine agar supplemented with 0.01% (wt/vol) l-arabinose. The data sets shown are representative of at least two independent experiments where similar growth patterns were observed. Due to the fragile nature of the overlay plates after incubation at high pressure, the plates could only be photographed in the plastic pouches. The imperfections in the photographs are due to deformations of the plastic pouches.