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The ability of the P. profundum SS9 PBPRA1039 and E. coli seqA genes to complement the morphological defect of an E. coli seqA mutant is dependent upon temperature. Cultures of the strains shown were diluted 1:500 in LB broth with thymine, streptomycin, and 0.01% (wt/vol) l-arabinose and grown to mid-exponential phase (OD600, ∼0.4) at the temperatures shown. The images shown are representative fields of view from at least two independent experiments. Scale bars = 10 μm.
