TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| P. profundum | ||
| SS9 | High-pressure- and cold-loving deep-sea isolate | 8 |
| SS9R | Rifr SS9 derivative | 7 |
| FL23 | SS9R PBPRA3229::mini-Tn10 Rifr Kanr | 19 |
| FL28 | SS9R PBPRA1039::mini-Tn10 Rifr Kanr | 19 |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | 13 |
| MM294A | pro-82 thi-1 endA1 supE44 hsdR17 | 12 |
| χ705 | F−leu-4 φr Strrarg-35 T6r λ− | 4 |
| χ711 | χ705 ΔgmhA Strr | 4 |
| KH5402-1 | ilv thyA thr tyrA trpE9829 metE deo supF6 | 15 |
| NA001 | KH5402-1 dnaA(Cs) | 16 |
| NA026 | NA001 diaA::Tn5 Kanr | 14 |
| NA141 | KH5402-1 diaA::Tn5 Kanr | 14 |
| BW25113 | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | 2 |
| JW0674 | BW25113 ΔseqA Kanr | 2 |
| ZE0674 | KH5402-1 ΔseqA Kanr | This study |
| Plasmids | ||
| pFL190 | Broad-host-range plasmid with PBAD inducible promoter, Strr | 18 |
| pECdiaA | pFL190 with entire E. coli diaA gene and 77 bp upstream | This study |
| pPBPRA3229 | pFL190 with entire PBPRA3229 gene and 70 bp upstream | This study |
| pECseqA | pFL190 with entire E. coli seqA gene and 81 bp upstream | This study |
| pPBPRA1039 | pFL190 with entire PBPRA1039 gene and 57 bp upstream | This study |
| pRK2013 | Helper plasmid for mating, Kanr | 9 |