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Alignment of oriT regions of IncQ-2 plasmids and the IncPα plasmid RP4, showing the sequence divergence that could be tolerated by the Mob proteins of plasmid pRAS3 while they were still able to mobilize DNA from an oriT. A vertical arrow indicates the relaxase nic site at which single-stranded cleavage takes place as determined for plasmid RK2/RP4 (29).
