TABLE 3.
Effects of the number of 6-bp repeats and 22-bp iterons on plasmid copy number
| Plasmid construct | No. of 6-bp repeats | No. of 22-bp iterons | Relative plasmid copy no.b | Calculated plasmid copy no.c |
|---|---|---|---|---|
| pRAS3.2 | 4 | 3 | 0.51 ± 0.09 | 30 ± 5 |
| pRAS3.1 | 5 | 4 | 0.69 ± 0.062 | 41 ± 4 |
| pRAS3.1.35a | 5 | 3 | 1.0 | 59 |
| pRAS3.1.34 | 4 | 3 | 0.53 ± 0.002 | 31 ± 1 |
| pRAS3.1.44 | 4 | 4 | 0.39 ± 0.037 | 23 ± 2 |
a
pRAS3.1.35 served as the reference for the determination of relative copy numbers and standard deviations.
b
The number of replicates for relative copy number determinations was four to six, and a P value of 0.001 was obtained for each qPCR experiment.
c
Plasmid numbers were calculated to the nearest whole plasmid.