Growth rates of the NagB constructs on GlcN and GlcNAc. LAA20 with pXEI-derived plasmids carrying the NagB-L1, -L2, -L3, and -L4 constructs expressing wt NagB to different levels, NagB-L4 with the F174A mutation, or MC4100 carrying pXE1 was grown on GlcN or GlcNAc. Growth on GlcN was also measured in the presence of the mutations mlc, ΔnagE-nagBACD, nagA, and nagC as indicated. The histogram gives the DT in minutes ± standard deviations and the means of four to eight cultures (note that the scales vary).