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Oligonucleotides used
| Oligonucleotide | Function | Sequencea |
|---|---|---|
| NagB70 | 5′ nagB | GACTTCTAGATTACTTATTGAGGGTGAATAATG |
| NagB71 | 5′ nagB with a high-expression S&D sequence | GACTTCTAGAAAAATAAGGAGGAAATAAAATG AGACTGATCCCCCTGAC |
| NagB72 | 3′ nagB | GACTGAATTCAGGAGCCAGGGCAGGGATAA |
| NagB73 | 3′ nagB with stem-loop structure | GACTGAATTCCGTTTAACTGCACATCGATA |
| XE1 | Sequence inserts pXE1 | GGTTGGCTCCAATTATTTGTATATTC |
| RBP22 | Sequence inserts pXE1 | CCGAAAAGTGCCACCTGACGTC |
Restriction sites used for cloning are underlined.
