FIG. 2.

PA pathway metabolites accumulated in surviving S. pyogenes CS101 cultures. S. pyogenes strain CS101 was grown in static TH broth cultures under a 5% CO₂ atmosphere. Culture samples were removed at various points after entrance into stationary phase. The samples were filter sterilized, and the concentrations of lactate, formate, acetate, and ammonia were determined. Metabolite concentrations present in sterile TH broth are represented by “TH”. Time is given in weeks under each bar, and T₀ (“0”) is entry into stationary phase. Each bar represents the mean value from at least four independent cultures. Error bars are the standard deviations of the means.