TABLE 1.
Oligonucleotides used in this study
| Name | Sequence (5′-3′) | Name | Sequence (5′-3′) |
|---|---|---|---|
| lctO FWD | AGCACCTGTAGCGGCTCATAAACT | ccpA REV | AGATGGTGCTCATAATTCAC |
| lctO REV | ATACCTGATGCTCCTGCGTCCAAT | srv FWD 1 | TACTATCAAAGGGCATTAGC |
| acoA FWD | GCTTGCTGGTAAAGCAACTGGTGT | srv FWD 2 | TGTATGTTAACTAACGGCCG |
| acoa REV | ATAGCTGGACCATTTCCACCACGA | srv REV | TGCAGATCCAGATCAAAGCC |
| pfl FWD | TCGTCTTGCTCTTTACGGTGCTGA | SPy1548 FWD | GGAACGTATGAGTTGGTGAC |
| pfl REV | TCTGGCAGTTGGTCAGTCCAAAGA | SPy1548 REV | TTCTTTACCGGGTCTGTGGAGC |
| pta FWD | TGACACTGTTCGTCCAGCTCTTCA | relA FWD 1 | ACTAAGCGCTTTCTTAGCAG |
| pta REV | GCCATCAAGTGCCAAATCAGGGTT | relA FWD 2 | TCGCATCAAATGGGAACTAG |
| ackA FWD | CGTGTTGTTGCTGGTGGTGAACTT | relA REV | TTGACCCGTTGCAAGACAAG |
| ackA REV | AAGTGGTGTAAAGCCCATCGAGGT | SPy0145 FWD 1 | TCAAGAGCAAAGGTGGTGAGAGGA |
| gyrA FWD | ACAGGTCGGGAACGTATTGTGGTT | SPy0145 FWD 2 | CCAGTGACAGGTCAATTGTC |
| gyrA REV | GTTCCAAACCAGTCAAACGACGCA | SPy0145 REV | GAGGCACAAGCTGCCGAAGC |
| codY FWD 1 | ACAAGCTAGTGCTTATCTCC | SPy1630 FWD | AGCTGAGCGGGTTAAGCGTATCAT |
| codY FWD 2 | TATCCAGGAGGTCTAACGAC | SPy1630 Rev | TGGCTTGTCCGTTTGCATCAATGG |
| codY REV | AAACAGGGAAACCTCTCCCC | CovRS FWD 1 | GATAGATTAAGAGGATAAGGGTTGGT |
| ropB FWD 1 | AAGCGACTATCATCCGAAAC | CovRS FWD 2 | TGTTTGGAAATATGATGAAGCCGT |
| ropB FWD 2 | ACTTGGAGTCACTATGAGAC | CovRS FWD3 | TGGTCCTATCGGTCGTGTGTATCA |
| ropB REV | AAGCTAACACCATAAGAGCG | CovRS FWD 4 | TGAGGCTGACCGTATGGCAATCAT |
| ccpA FWD 1 | AAAGTGGTTACAAATCATGC | CovRS REV | CATCAGCTTCTAACCAGTTGTGGC |
| ccpA FWD 2 | ACGCTCTCGTACTCCAGTTG |