FIG. 2.

Influence of catalase and H₂O₂ addition on eDNA amounts of S. sanguinis (Ss) and S. gordonii (Sg) cultures. (A) The relative eDNA amounts in culture supernatants were measured with real-time PCR and standardized to 16S rRNA of isolated chDNA by using different dilutions. Data presented are the means ± SD of results from two independent experiments done on different days. (B) Effect of H₂O₂ addition on eDNA release of the Pox⁻ mutants. Semiquantitative PCR was performed on precipitated eDNA. (C) Complementation of the Pox⁻ mutants reinstalled eDNA release. Semiquantitative PCR was performed on precipitated eDNA. Pictures are representatives of at least two experiments. WT, wild type.