FIG. 2.

Confirmation of microarray results using real-time RT-PCR. RNA was isolated from the wild-type strain, 6939 (white bars), an Rbf overexpression strain, 6933 (gray bars), and an rbf mutant strain, 6970 (black bars) in flow cell cultures and subjected to real-time RT-PCR using primers specific for the indicated genes (Table 3). Expression levels are expressed relative to that of strain 6939, which was arbitrarily assigned a value of 1. The expression of gyrB was used to correct for differences in RNA quantities added to each reaction mixture. Data represent the means ± standard errors from two independent experiments.