FIG. 5.

Contributions of rbf and icaR to biofilm formation by 8325-4. (A) Biofilm formation under static incubation conditions. Microtiter plate wells were inoculated with each of the indicated 8325-4 derivatives. Following a 24-h incubation, wells were washed and biofilms were stained with crystal violet. Each assay was performed a minimum of two times. (B) Quantitation of biofilms. Crystal violet was extracted from wells by using ethanol-acetic acid and diluted 10-fold, and the absorbance of each extract was measured. Error bars indicate standard errors.