Transcriptional analyses of the ybaW gene and neighboring loci. (A) Genetic organization of the ybaW gene and the neighboring loci. The gray arrows represent open reading frames, and the small, numbered arrows correspond to the primers. 1, ybaV-F; 2, ybaV-R; 3, ybaW-F; 4, ybaW-R; 5, queC-F; 6, queC-R (Table 2). The primer combinations 1/2, 3/4, 5/6, 1/4, and 3/6 are shown. S denotes the transcriptional start site. The hairpin indicates a predicted terminator. The primer pairs used are shown above the brackets. (B) Results of PCR and RT-PCR (RT) analyses of the ybaW gene and its two neighboring genes.