FIG. 3.

Mapping of the ybaW transcriptional start site. (A) Result of 5′-RACE-based mapping of the transcription start site of the ybaW gene. The sequencing profile of the product is shown. The AAP sequence is within the rectangle. The same start site was found in the wild-type strain in the presence or absence of oleate induction and in a ΔfadR strain. RLM-RACE analyses (data not shown) also gave the same start site. The site identified was identical to that predicted by the Neutral Program of Promoter Prediction (http://www.fruitfly.org/seq_tools/promoter.html). (B) Sequence alignments of ybaW promoter regions in E. coli and Salmonella enterica. The bracketed sequence is the putative FadR binding site. S denotes the transcriptional start site, and M denotes the ybaW initiation codon. Black and gray shading shows identity and similarity, respectively. Boxes highlight differences, and dots show deletions relative to the other sequence.