FIG. 7.

Induction of fadM transcription. The cultures grown without a fatty acid carbon source were grown on glucose. (A) β-Galactosidase activities of a chromosomal fabM-lacZ transcriptional fusion strain grown on different fatty acids. (B) RT-qPCR analyses of fabM expression induced by oleate. The level of expression of fabA, which is known to be repressed upon the addition of oleate (25), serves as a control. Each sample was assayed in triplicate. (C) Effects of CLA on fad gene expression. (D) Effects of glucose on fad gene expression. The data are the means of the results of three independent assays. Levels of β-galactosidase activity are shown in Miller units, and error bars show standard deviations.