TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| MG1655 | Wild-type K-12 strain | Laboratory stock |
| UB1005 | metB1 spoT1 relA1 spoT1 gyrA216 | 25 |
| MFH8 | UB1005 fadR::Tn10 | 25 |
| MFH9 | UB1005 fadR::Tn5 | 25 |
| SI91 | UB1005 fabR::Cm Cmr | Laboratory stock |
| MC1061 | Δ(codB-lacI)3 | 6 |
| Topo10 | lacX74 | Invitrogen |
| BL21(DE3) | Expression host for protein production | Laboratory stock |
| MC4100 | araD139 Δ(argF-lac)169 | Laboratory stock |
| RH74 | MC4100 Δcya-851 | Laboratory stock |
| RH77 | MC4100 Δcya-851 Δcrp | Laboratory stock |
| SI203 | MC1061 fadBA-lacZ fusion | Laboratory stock |
| LE392 | Wild-type strain | 9 |
| HC70 | LE392 tesA::Km tesB::Cm | 9 |
| FYJ14 | MC1061 ybaW::Km | This work |
| FYJ15 | MC1061 ΔybaW | This work |
| FYJ16 | FYJ15 carrying pCP20 | This work |
| FYJ17 | FYJ15 ybaW-lacZ fusion | This work |
| FYJ18 | FYJ17 fadR::Tn10 | This work |
| FYJ19 | FYJ17 fabR::Cm | This work |
| FYJ20 | MC4100 ybaW-lacZ fusion | This work |
| FYJ21 | RH74 ybaW-lacZ fusion Δcya-851 | This work |
| FYJ22 | RH77 ybaW-lacZ fusion Δcya-851 Δcrp | This work |
| FYJ23 | FYJ20 fadR::Tn10 | This work |
| Plasmids | ||
| pCR2.1-TOPO | Topo cloning vector | Invitrogen |
| pKD46 | λred recombinase plasmid, temperature sensitive | 15 |
| pKD4 | bla FRT ahp FRT oriR6K | 15 |
| pCP20 | bla cat cI857 λPRflp oriTS | 7, 19 |
| pCE70 | ahp FRT lacZY+ thisoriR6K | 19 |
| pET28-fadRec | Expression plasmid encoding E. coli fadR | This work |
| pET28-fabRec | Expression plasmid encoding E. coli fabR | This work |