E. coli strains |
|
|
MG1655 |
Wild-type K-12 strain |
Laboratory stock |
UB1005 |
metB1 spoT1 relA1 spoT1 gyrA216 |
25 |
MFH8 |
UB1005 fadR::Tn10
|
25 |
MFH9 |
UB1005 fadR::Tn5
|
25 |
SI91 |
UB1005 fabR::Cm Cmr
|
Laboratory stock |
MC1061 |
Δ(codB-lacI)3
|
6 |
Topo10 |
lacX74
|
Invitrogen |
BL21(DE3) |
Expression host for protein production |
Laboratory stock |
MC4100 |
araD139 Δ(argF-lac)169
|
Laboratory stock |
RH74 |
MC4100 Δcya-851
|
Laboratory stock |
RH77 |
MC4100 Δcya-851 Δcrp
|
Laboratory stock |
SI203 |
MC1061 fadBA-lacZ fusion |
Laboratory stock |
LE392 |
Wild-type strain |
9 |
HC70 |
LE392 tesA::Km tesB::Cm |
9 |
FYJ14 |
MC1061 ybaW::Km |
This work |
FYJ15 |
MC1061 ΔybaW
|
This work |
FYJ16 |
FYJ15 carrying pCP20 |
This work |
FYJ17 |
FYJ15 ybaW-lacZ fusion |
This work |
FYJ18 |
FYJ17 fadR::Tn10
|
This work |
FYJ19 |
FYJ17 fabR::Cm |
This work |
FYJ20 |
MC4100 ybaW-lacZ fusion |
This work |
FYJ21 |
RH74 ybaW-lacZ fusion Δcya-851
|
This work |
FYJ22 |
RH77 ybaW-lacZ fusion Δcya-851 Δcrp
|
This work |
FYJ23 |
FYJ20 fadR::Tn10
|
This work |
Plasmids |
|
|
pCR2.1-TOPO |
Topo cloning vector |
Invitrogen |
pKD46 |
λred recombinase plasmid, temperature sensitive |
15 |
pKD4 |
bla FRT ahp FRT oriR6K
|
15 |
pCP20 |
bla cat cI857 λPRflp oriTS
|
7, 19
|
pCE70 |
ahp FRT lacZY+ thisoriR6K
|
19 |
pET28-fadRec |
Expression plasmid encoding E. coli fadR
|
This work |
pET28-fabRec |
Expression plasmid encoding E. coli fabR
|
This work |