TABLE 2.

Primers used in this study

Primer	Sequence
ybaW-F	5′-CAT CTC GAC GTT TAC CAG CAC G-3′
ybaW-R	5′-GTT AAC AGG TCA CTT AAT ACC GCT G-3′
ybaW_P-F	5′-TCT GGC GGT ATT AAC CCT GT-3′
ybaW_P-R	5′-GTG TTT GCA TAG CGC AAT AAC-3′
16S-F	5′-GCA TAA CGT CGC AAG ACC AAA G-3′
16S-R	5′-TTC TTC ATA CAC GCG GCA TGG-3′
ybaW-F2	5′-AGC TGG AGC AGA TGG TTA AG-3′
ybaW-GSP	5′-TTA CTT AAC CAT CTG CTC CAG-3′
AAPa	5′-GGC CAC GCG TCG ACT AGT ACG GGI IGG GII GGG IIG-3′
AUAP	5′-GGC CAC GCG TCG ACT AGT AC-3′
RLM-RACE Adaptor	5′-GCU GAU GGC GAU GAA UGA ACA CUG CGU UUG CUG GCU UUG AUG AAA-3′
RLM-RACE Outer	5′-GCT GAT GGC GAT GAA TGA ACA CTG-3′
RLM-RACE Inner	5′-CGC GGA TCC GAA CAC TGC GTT TGC TGG CTT TGA TG-3′
ybaW-check1	5′-CAA CAA CGT AAG GTT ATT GCG C-3′
ybaW-check2	5′-AG CAT CCG GCA CCA CAA AAC-3′
ybaV-F	5′-AGC ACT GCT CAT TAC CCT GTC-3′ (18-38)
ybaV-R	5′-CAG TTT TAA ACG GAC CGT ACT C-3′ (277-298)
queC-F	5′-GGG CAT GGC GAA AGA TAT TCG-3′ (444-464)
queC-R	5′-CTC AAC CCG GTT TTC TGC TTC-3′ (678-693)
ybaW_fusion-F	5′-TAC CAG TTA TGA CCT CTG TAC TTA TAA CAA CAA CGT AAG GTT ATT GCG CT TGT GTA GGC TGG AGC TGC TT-3′
ybaW_fusion-R	5′-TAT TCC GGG TGT CGC CGG ATG CGG CTT GAG CAT CCG GCA CCA CAA AAC GT CAT ATG AAT ATC CTC CTT AG-3′

^aThe letter “I” in the AAP primer denotes deoxyinosine, which is included in an improved anchor primer to eliminate the need for the mixture of anchor and adaptor primers used in the previous 5′-RACE version. Numbers in parentheses are the locations of the primers within the coding sequence of the gene of interest.