TABLE 4.
Inhibition of fadM transcription by the cAMP-CRP complexa
| Strain | Transcriptional level of fadM in indicated assay
|
|
|---|---|---|
| LacZ activity | RT-qPCR | |
| Wild type | 1.00 ± 0.07 | 1.00 ± 0.05 |
| ΔcyaA strain | 3.65 ± 0.31 | 5.68 ± 0.66 |
| ΔcyaA Δcrp strain | 2.07 ± 0.27 | 3.65 ± 0.38 |
a
Data are shown relative to the results for the wild type. LacZ activity was measured as β-galactosidase activity; the activity of the wild-type strain was 138.5 ± 10 Miller units. The β-galactosidase activities are the means ± standard deviations of the results of three independent assays, whereas each sample of the RT-qPCR experiments was analyzed in triplicate. The strains used for LacZ activity measurements were FYJ20, FYJ21, and FYJ22, whereas those used for RT-qPCR analyses were MC4100, RH74, and RH77.