TABLE 5.
Effect of exogenous cAMP on fadM expression measured using fadM-lacZ fusion strainsa
| Medium | cAMP | β-Galactosidase activity (Miller units) in:
|
|||
|---|---|---|---|---|---|
| Wild type | ΔcyaA strain | ΔcyaA Δcrp strain | ΔfadR strain | ||
| RB | − | 131 ± 11 | 387 ± 61 | 240 ± 40 | 351 ± 43 |
| M9 | − | 130 ± 12 | 363 ± 35 | 256 ± 14 | 364 ± 38 |
| M9 | + | 97 ± 5 | 117 ± 7 | 239 ± 14 | 277 ± 21 |
a
The cultures were grown in either RB medium or glucose minimal medium (M9). Growth in the latter medium was either in the absence (−) or presence (+) of 1 mM cAMP. The β-galactosidase activities are the means ± standard deviations of the results of three independent assays. The strains used for LacZ activity measurements were FYJ20, FYJ21, FYJ22, and FYJ23.