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. 2009 Aug 7;75(19):6124–6131. doi: 10.1128/AEM.00809-09

FIG. 2.

FIG. 2.

Identification of transcriptional start sites for sodC and rpoE in A. pleuropneumoniae by 5′ rapid amplification of cDNA ends. (A) Sequence showing the transcriptional start site for sodC (bold) and the predicted −10 and −35 promoter regions (underlined). The −10 sequence agrees with that previously predicted by Langford et al. (28) using primer extension. In that study, the −35 region was predicted to be TTATT, although an alternative (TTTAAA), which shows closer homology to the consensus for the −35 region of E. coli σ70 promoters (TTGACA), is present. (B) Sequence showing the transcriptional start site for rpoE (bold) and the predicted −10 and −35 promoter regions (underlined). The predicted −35 region is a perfect match for the −35 region of the E. coli σE promoter (GAACTT), whereas the −10 region (ACTAA) differs slightly from that in E. coli (TCAAA). For both sodC and rpoE, the boxed regions show the beginning of the translated genes.