FIG. 5.

Expression of GFP in A. pleuropneumoniae S4074 bacteria containing different reporter plasmids. The relative fluorescence of the bacteria, grown either to stationary phase on BHI-Lev plates or to mid-log phase in BHI broth, was measured on gated populations of bacterial cells by flow cytometry, producing data in relative light units (RLU) per cell. In each case, the background fluorescence from A. pleuropneumoniae S4074 containing the pMC-Tandem (promoterless) has been subtracted. Experiments were performed in triplicate, and the error bars indicate the standard deviations of the means.