FIG. 7.

Induction of the A. pleuropneumoniae sodC and rpoE promoters following exposure to ethanol. The level of fluorescence per cell was measured following addition of 3% (vol/vol) ethanol to mid-log phase cultures (at 0 h). In A. pleuropneumoniae S4074 containing pMC-Tandem (promoterless), the levels of fluorescence remained constant throughout the time course, while the levels of fluorescence in cells containing pMCsodCP or pMCrpoE increased over time. Following addition of ethanol, viable cell counts did not increase, and by 5 h there was a 10-fold reduction in viable cell counts. Experiments were performed in triplicate, and the error bars indicate the standard deviations of the means.