FIG. 3.

Schematic diagram of the engineered 563-bp gat gene on pwFRT-PC_S12-gat. The B. cenocepacia rpsL promoter (PC_S12) and ribosomal binding site (rbs) are shown in relation to the gat gene. Below the schematic are the corresponding nucleotide and protein sequences. Codons were optimized according to the codon preference within the B. pseudomallei K96243 asd gene. Also indicated are the −35 and −10 regions of the PC_S12 promoter. Restriction sites (in boldface) were positioned strategically for subsequent cloning and manipulation.