TABLE 1.
Bacterial strains used in this studya
| Strain | Lab IDb | Relevant properties | Source |
|---|---|---|---|
| E. coli | |||
| K-12 | E0577 | Wild type; F+ | Coli Genetic Stock Center |
| EPMax10B-pir116/Δasd::Gmr | E1345 | Gmr; F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::Gmr-wFRT | —c |
| EPMax10B-lacIq/pir/leu+/Δasd::Gmr | E1951 | Gmr; F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 galU galK rpsL nupG lacIq-FRT8 pir::FRT4 Δasd::Gmr-wFRT | —c |
| EPMax10B-pir116/Δasd/Δtrp::Gmr/ mob-Kmr | E1354 | Gmr Kmr; F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG Tn-pir116-FRT2 Δasd::wFRT Δtrp::Gmr-FRT5 mob[recA::RP4-2 Tc::Mu-Kmr] | —c |
| EPMax10B-lacIq/pir | E1869 | F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK rpsL nupG lacIq-FRT8 pir-FRT4 | —c |
| EPMax10B-lacIq/pir/leu+ | E1889 | F− λ−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 galU galK rpsL nupG lacIq-FRT8 pir-FRT4 | —c |
| GM33 | E0021 | F−λ− IN(rrnD-rrnE)1 dam-3 sup-85 | 32 |
| Burkholderia spp. | |||
| B. dolosa AU0158 | E1551 | Prototroph | J. Goldberg |
| B. cenocepacia | |||
| Bc7 | E1552 | Prototroph | J. Goldberg |
| K56-2 | E1554 | Prototroph; cystic fibrosis isolate | P. Sokol |
| B. mallei ATCC 23344 | B0002 | Wild-type strain; postmortem isolate | 34 |
| B. pseudomallei | |||
| 1026b | B0004 | Wild-type strain; clinical melioidosis isolate | 13 |
| 1026b-ΔasdBp::gat-FRT | B0011 | GSr; 1026b with a gat-FRT cassette inserted into the asdBp gene | This study |
| 1026b-ΔasdBp::gat-FRT/attTn7-bar-asdBp | B0015 | GSr PPTr; 1026b ΔasdBp::gat-FRT mutant with mini-Tn7-bar-asdBp inserted | This study |
| 1026b-ΔdapBBp::gat-FRT | B0013 | GSr; 1026b with a gat cassette inserted into the dapBBp gene | This study |
| K96243 | B0002 | Wild-type strain; clinical melioidosis isolate | 23 |
| K96243-ΔasdBp::gat-FRT | B0007 | GSr; K96243 with a gat cassette inserted into the asdBp gene | This study |
| K96243-ΔdapBBp::gat-FRT | B0010 | GSr; K96243 with a gat cassette inserted into the chromosomal dapBBp gene | This study |
| B. thailandensis E264 | E1298 | Prototroph; environmental isolate | 4 |
a
Abbreviations and designations: bar, gene encoding bialaphos (PPT) resistance; gat, gene encoding GAT; Gmr, gentamicin resistant; GSr, glyphosate resistant; Kmr, kanamycin resistant; PPTr, PPT resistant.
b
Please use the laboratory identification number (lab ID) when requesting strains.
c
Details on the engineering of this strain are to be published elsewhere.