TABLE 3.
Summary of phylogenetic similarities determined from sequences obtained for dominant bands in DGGE profiles and isolates from microcosms amended with organic C (glucose or protein), NO3−, and 240 μg Cr(VI) g−1 soila
| Clone or isolateb | Best match | Accession no. | Similarityc (% similarity) | Phylogenetic clade |
|---|---|---|---|---|
| GNM-1(a) | Carnobacteriaceae clone BLUC-K | DQ196615 | 196/197 (99) | Firmicutes |
| GNM-2(b) | Bacterium clone FI-2F_H03 | EF220454 | 168/173 (97) | Cytophaga-Flavobacterium-Bacteroides group |
| GNM-3(b) | Uncultured Aranicola sp. strain LJEr1 | EU789569 | 197/197 (100) | Gammaproteobacteria |
| GNM-4(b) | Bacterium clone FFCH4639 | EU135403 | 169/169 (100) | Candidate division TM7 |
| GNM-5(b) | Serratia liquefaciens strain ZY-2 | EU880537 | 197/197 (100) | Gammaproteobacteria |
| GNM-6(b) | Uncultured bacterium clone 70 | DQ165123 | 165/169 (97) | Candidate division TM7 |
| GNM-7(c) | Bacterium clone FFCH10465 | EU134917 | 173/173 (89) | Candidate division OP10 |
| GNM-8(c) | Serratia sp. strain Tp5 | EU855752 | 197/197 (100) | Gammaproteobacteria |
| GNM-9(d) | Uncultured bacterium clone C08.ab1 | EU136313 | 174/174 (100) | Actinobacteria |
| GNM-10(e) | Propionibacterium freudenreichii ISU P59 | AY533300 | 171/174 (98) | Actinobacteria |
| Isolate GNCr-1 | Cellulosimicrobium sp. strain 87N50-1 | EU196469 | 455/456 (99) | Actinobacteria |
| Isolate GNCr-2 | Lactosphaera pasteurii | X87150 | 1477/1563 (94) | Firmicutes |
| Isolate GNCr-3 | Bacillus sp. strain CNJ940 PL04 | DQ448802 | 533/533 (100) | Firmicutes |
| Isolate GNCr-4 | Bacillus sp. strain cp-h51 | EU558977 | 536/536 (100) | Firmicutes |
| PNM-1(f) | Antarctic bacterium strain GA0A | EU636049 | 197/197 (100) | Gammaproteobacteria |
| PNM-2(g) | Brevibacillus brevis ZFJ-2 16S | EU931557 | 198/198 (100) | Firmicutes |
| PNM-3(h) | Aranicola clone LJEr1 | EU789569 | 197/197 (100) | Gammaproteobacteria |
| PNM-4(i) | Serratia sp. strain Tp5 | EU855752 | 197/197 (100) | Gammaproteobacteria |
| PNM-5(i) | Chlamydiales bacterium P-5 | AF364565 | 187/197 (94) | Chlamydiae |
| PNM-6(j) | Clone 37_C1_RHIZO_T7s | EF605981 | 172/172 (100) | Alphaproteobacteria |
| GNM-9(d) | Uncultured bacterium clone C08.ab1 | EU136313 | 174/174 (100) | Actinobacteria |
| PNM-8(l) | Mycobacterium sp. strain AT-3 | AF220427 | 177/177 (100) | Actinobacteria |
| Isolate PNCr-1 | Bacillus thuringiensis 2PR56-10 | EU440975 | 515/521 (98) | Firmicutes |
| Isolate PNCr-2 | Bacillus sp. strain PK-9 | EU685818 | 485/485 (100) | Firmicutes |
| Isolate PNCr-3 | Bacillus sp. strain ERI 44 | EU984074 | 422/424 (99) | Firmicutes |
| Isolate PNCr-4 | Bacterium GFCr-1 | DQ154277 | 499/504 (99) | Actinobacteria |
| Isolate PNCr-5 | Bacterium GFCr-1 | DQ154277 | 502/504 (99) | Actinobacteria |
a
All sequences are available in GenBank under accession numbers DQ426690 to DQ426716.
b
Clones and isolates starting with the capital letter G were from glucose-amended microcosms; clones and isolates starting with the capital letter P were from protein-amended microcosms.
c
The values shown are the number of similar base pairs/number of total base pairs. Approximately 200 bp was sequenced for clones and 500 bp for isolates.