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Confirmation of pLgLA39. (A) Agarose gel electrophoresis. Lanes: M, molecular size marker (λ HindIII digest); 1, total DNA preparation from LA39; 2, purified pLgLA39 plasmid; 3, plasmid preparation from JCM 1131T(pLgLA39/pSYE2); 4, pSYE2. (B) Southern hybridization. Lanes: 1 and 3, total DNA preparation from LA39; 2 and 4, purified pLgLA39 plasmid. The probes used were the gaaA probe (see Fig. 2) for lanes 1 and 2 and the 16S rRNA gene probe for lanes 3 and 4.
