FIG. 2.

Physical and genetic map of pLgLA39. Genes for gassericin A production, for conjugation, and for replication and maintenance are shown as black, shaded, and hatched arrows, respectively. Open and shaded boxes indicate the DNA regions used for construction of the plasmids and those used as the hybridization probe, respectively. A closed box indicates the supposed oriT sequence. The repeat region in repA is also indicated. Predicted terminators are shown by Ω-like symbols (upside-down ones are to terminate leftward transcriptions). Sites for rare-cutting restriction endonucleases are shown with total site numbers in parentheses.