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. 2009 Aug 7;75(19):6340–6351. doi: 10.1128/AEM.00195-09

FIG. 4.

FIG. 4.

Effect of gassericin A production on apparent plasmid segregational stability. (A) gaa expression plasmid pGAA and gaaA-deletant plasmid pGAAΔgaaA. Primers used in construction of pGAAΔgaaA are shown. The double-lined region is from pLgLA39. ermA indicates the 1,111-bp HhaI fragment of pIL253 (GenBank accession number AF041239) containing the Em resistance (adenine methylase) gene. repA_pSY indicates the replication initiator protein gene of pSY1 (GenBank accession number E05086). (B) Apparent segregational stabilities of plasmids in L. gasseri JCM 1131T. Symbols: circles, pGAA; squares, pGAAΔgaaA; triangles, pSYE2.