TABLE 2.
Primers used
| Primer name | Sequence (5′ to 3′)a | Location (nt) or descriptionb | Reference |
|---|---|---|---|
| pr01 | ATGGTTACTAAGTACGGACG | 2717-2736 | |
| pr02 | CTAGGCTGCAGTCGCTCCCA | 2992-2973 | |
| pr03 | CCGCAGATAGGCTAGGTGTC | 1624-1643 | |
| pr04 | TAATTCCTAGTATAGCACAG | 5883-5864 | |
| pr05 | AAAACTCGAGAGTATCTTCCTCCCATAGG | AAAA plus XhoI site plus nt 2721 to 2703 | |
| pr06 | AAAACTCGAGTGATTATAAAGCTTTATGAATAG | AAAA plus XhoI site plus nt 2998 to 3020 | |
| pr07 | CCTCGCAAGGGAAAAGACAG | 278-297 | |
| pr08 | GCAGAGCAACTGCTGGCGAG | 1268-1249 | |
| pr09 | CTTCGTAACAATGCTATGAC | 718-699 | |
| pr10 | ATGACAAAATCAACAGATTTC | 12632-12652 | |
| pr11 | TTTAAAAGCCCCTATTCTTC | 13841-13822 | |
| pr_q01 | CAGCTATGGGAAACGACTATGAA | 6164-6186 (ATCC 33323 genome sequence) | 29 |
| pr_q02 | CGTATTCTGTGATCTTGAGGTTG | 6382-6360 (ATCC 33323 genome sequence) | 29 |
| pr_q03 | CAACAACTGGGTTGATGAAGAAG | 12799-12821 | |
| pr_q04 | AAATAGACATCGGTTGCTTTCAC | 13026-13004 | |
| pr_q05 | TACCTTGGATATTCACCGAACAC | 501-479 (pSYE2) | 42 |
| pr_q06 | GTTGACGATATTCTCGATTGACC | 287-309 (pSYE2) | 42 |
| 27F | AGAGTTTGATC(A/C)TGGCTCAG | Used to amplify 16S rRNA gene | 25 |
| 1492R | TACGG(C/T)TACCTTGTTACGACTT | Used to amplify 16S rRNA gene | 25 |
a
The XhoI site is underlined.
b
Numbers indicate coordinates in the nucleotide sequence of pLgLA39, unless otherwise indicated.