TABLE 3.
Characteristics of ORFs and predicted proteins in pLgLA39
| ORF | Location (nt) | Deduced function | Protein length (aa) | pI | Mol wt | COG | Cellular locationa |
|---|---|---|---|---|---|---|---|
| 01 | 269-93 | Hypothetical protein | 58 | 7.94 | 6,658.7 | Soluble | |
| 02 | 366-611 | PemI (inhibitor) | 81 | 5.43 | 9,250.5 | COG2336 | Soluble |
| 03 | 605-937 | PemK (RNase toxin) | 110 | 9.58 | 12,564.7 | COG2337 | Soluble |
| 04 | 1871-2395 | GaaB | 174 | 9.60 | 20,278.4 | Membrane (5) | |
| 05 | 2433-2615 | GaaC | 60 | 9.61 | 7,290.9 | Membrane (2) | |
| 06 | 2722-2997 | GaaA (gassericin A) | 91 | 9.40 | 9,286.0 | COG1300 | Membrane (2) |
| 07 | 3081-3569 | GaaD | 162 | 6.94 | 18,344.2 | Membrane (4) | |
| 08 | 3591-3752 | GaaI (immunity) | 53 | 11.10 | 6,134.5 | Membrane (1) | |
| 09 | 3775-4455 | GaaT (transporter) | 226 | 4.71 | 25,126.0 | COG1131 | Soluble |
| 10 | 4458-5096 | GaaE (transporter) | 212 | 9.51 | 23,665.0 | Membrane (6) | |
| 11 | 5560-5246 | LtrC (fragment) | 104 | 9.87 | 12,323.9 | Soluble | |
| 12 | 5923-6507 | Resolvase | 194 | 9.99 | 22,341.8 | COG1961 | Soluble |
| 13 | 7030-6599 | Transposase (fragment) | 143 | 8.75 | 16,163.5 | COG3293 | Membrane (1) |
| 14 | 7374-7003 | Transposase (fragment) | 123 | 9.47 | 14,365.4 | COG3293 | Soluble |
| 15 | 9491-7650 | Multidrug resistance transporter | 613 | 9.97 | 65,539.8 | COG0477 | Membrane (4) |
| 16 | 9656-10240 | Regulator | 194 | 6.07 | 22,906.3 | COG1309 | Soluble |
| 17 | 10325-10633 | Resolvase (fragment) | 102 | 10.22 | 12,215.2 | COG1961 | Soluble |
| 18 | 10765-11814 | StbA homolog | 349 | 5.16 | 39,098.0 | COG0443 | Soluble |
| 19 | 11817-12092 | Hypothetical protein | 91 | 5.82 | 10,228.7 | Soluble | |
| 20 | 12632-13741 | RepA | 369 | 6.16 | 42,999.6 | Soluble | |
| 21 | 13984-14265 | RelB antitoxin | 93 | 4.66 | 10,483.9 | COG3077 | Soluble |
| 22 | 14312-14497 | Conserved hypothetical protein | 61 | 5.29 | 7,438.5 | Soluble | |
| 23 | 14494-15174 | Conserved hypothetical protein | 226 | 9.17 | 25,968.5 | Soluble | |
| 24 | 15442-15164 | Conserved hypothetical protein | 92 | 9.94 | 11,146.7 | Soluble | |
| 25 | 15674-15465 | Conserved hypothetical protein | 69 | 9.30 | 7,971.1 | Soluble | |
| 26 | 15946-18009 | TraA (nicking enzyme) | 687 | 8.92 | 80,624.2 | Soluble | |
| 27 | 18094-18405 | Conserved hypothetical protein | 103 | 9.63 | 11,687.6 | Soluble | |
| 28 | 18442-19056 | Conserved hypothetical protein | 204 | 5.11 | 23,696.4 | Soluble | |
| 29 | 19058-19393 | TraB | 111 | 9.21 | 11,717.0 | Membrane (3) | |
| 30 | 19414-19776 | TraC | 120 | 9.95 | 13,862.6 | Membrane (2) | |
| 31 | 19745-20404 | TraD | 219 | 5.46 | 24,877.5 | Soluble | |
| 32 | 20416-22434 | TraE | 672 | 5.50 | 77,233.8 | COG1419 | Soluble |
| 33 | 22427-23845 | TraF | 472 | 9.10 | 53,277.4 | Membrane (1) | |
| 34 | 23846-25003 | Conserved hypothetical protein | 385 | 6.66 | 41,739.7 | COG0791 | Membrane (1) |
| 35 | 25017-25634 | Conserved hypothetical protein | 205 | 9.78 | 23,256.2 | Membrane (1) | |
| 36 | 25621-25989 | Conserved hypothetical protein | 122 | 9.74 | 14,199.4 | COG0526 | Membrane (1) |
| 37 | 25990-26460 | TraJ | 156 | 8.94 | 17,613.5 | Membrane (2) | |
| 38 | 26462-28018 | TraK | 518 | 5.38 | 58,268.6 | COG3505 | Soluble |
| 39 | 28033-28440 | Conserved hypothetical protein | 135 | 9.71 | 14,964.2 | Membrane (1) | |
| 40 | 28442-29281 | TraL | 279 | 8.58 | 31,155.0 | Membrane (6) | |
| 41 | 29296-29700 | Hypothetical protein | 134 | 9.27 | 16,000.8 | Membrane (2) | |
| 42 | 29715-31853 | TraI (topoisomerase) | 712 | 9.30 | 80,062.9 | COG0550 | Soluble |
| 43 | 31976-32191 | Conserved hypothetical protein | 71 | 4.14 | 7,826.8 | Soluble | |
| 44 | 32195-33319 | LtrC | 374 | 7.79 | 42,313.5 | Soluble |
a
Deduced by using the SOSUI web service (http://bp.nuap.nagoya-u.ac.jp/sosui/). Numbers of predicted transmembrane segments are shown in parentheses.