TABLE 1.

Target genes, primer designations, DNA sequences, PCR conditions, and PCR product sizes used for detection of stx genes and other STEC virulence markers

Target gene	Primer	Nucleotide sequence (5′-3′)	PCR conditions	PCR product size (bp)	Reference
stxB1	KS7	CCCGGATCCATGAAAAAAACATTATTAATAGC	94°C, 30 s; 52°C, 60 s; 72°C, 60 s	285	62
	KS8	CCCGAATTCAGCTATTCTGAGTCAACG
stxA2	LP43	ATCCTATTCCCGGGAGTTTACG	94°C, 30 s; 57°C, 60 s; 72°C, 90 s	584	19
	LP44	GCGTCATCGTATACACAGGAGC
eaea	SK1	CCCGAATTCGGCACAAGCATAAGC	94°C, 30 s; 52°C, 60 s; 72°C, 60 s	863	61
	SK2	CCCGGATCCGTCTCGCCAGTATTCG
ehxA	HlyA1	GGTGCAGCAGAAAAAGTTGTAG	90°C, 30 s; 57°C, 60 s; 72°C, 90 s	1,550	59
	HlyA4	TCTCGCCTGATAGTGTTTGGTA
espP	espP-A	AAACAGCAGGCACTTGAACG	94°C, 30 s; 56°C, 60 s; 72°C, 150 s	1,830	14
	espP-B	GGAGTCGTCAGTCAGTAGAT
nleA	V83-rev2	CTTCCATCGCACGTATATCAGC	94°C, 30 s; 58°C, 60 s; 72°C, 90 s	959-1,112	22
	V83-for2	ACAGCAACATGCACCGGAATGC	94°C, 30 s; 55°C, 60 s; 72°C 90s	1,015-1,168
	V83-rev3	GATATCGATGACCACATCTTCAGG
subAB	RTsubABF	GCAGATAAATACCCTTCACTTG	94°C, 30 s; 56°C, 30 s; 72°C, 42 s	232	52
	RTsubABR	ATCACCAGTCCACTCAGCC
cif	cif-int-s	AACAGATGGCAACAGACTGG	94°C, 30 s; 57°C, 60 s; 72°C, 30 s	383	44
	cif-int-as	AGTCAATGCTTTATGCGTCAT
cdt-V	cdtV-F	TTCATTGTTCGCCTCCTG	94°C, 60 s; 50°C, 60 s; 72°C, 60 s	755	20
	cdtV-R	TTTATAAGCTGGTATCCTG
efa1	88AT	AAGGTGTTACAGAGATTA	94°C, 60 s; 51°C, 60 s; 72°C, 60 s	266	50
	88TN	TGAGGCGGCAGGATAGTT
iha	iha-I	CAGTTCAGTTTCGCATTCACC	94°C, 30 s; 56°C, 60 s; 72°C, 90 s	1,305	64
	iha-II	GTATGGCTCTGATGCGATG

^aPrimers SK1 and SK1 are universal eae primers and detect all eae variants.