TABLE 2.
Whole-cell fatty acid compositions of C. jejuni 2097e48 and 11168 and E. coli K-12 before and after exposure to 6°C for 24 ha
| Strain (group) | % Fatty acid (mean ± SD)
|
% Fatty acid (mean ± SD)
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 12:0 | 13:0 | 14:0 | 15:0 | 16:0 | 16:1 | 17:0 | Δ17:0 | 18:0 | t18:1 | 18:1n-9 | 18:1n-7 | c9, 10 Δ19:0 | c11, 12 Δ19:0 | 18:3n-3 | 20:3n-6 | 20:4n-6 | |
| C. jejuni 2097e48 (control) | 0.91 ± 0.08 | 0.16 ± 0.20 | 17.80 ± 0.40 | 0.17 ± 0.01 | 36.63 ± 0.17 | 7.97 ± 0.17 | 0.07 ± 0.01 | 0.17 ± 0.03 | 1.20 ± 0.18 | 0.08 ± 0.03 | 0.30 ± 0.06 | 22.91 ± 0.80 | 0.16 ± 0.02 | 8.97 ± 0.10 | 0.03 ± 0.02 | ND | ND |
| C. jejuni 2097e48 (chill) | 0.93 ± 0.05 | 0.03 ± 0.00 | 17.41 ± 0.40 | 0.19 ± 0.02 | 35.96 ± 0.19*** | 8.05 ± 0.07 | 0.09 ± 0.01* | 0.20 ± 0.02 | 1.46 ± 0.30 | 0.10 ± 0.01 | 0.36 ± 0.10 | 23.25 ± 0.51 | 0.17 ± 0.05 | 8.96 ± 0.52 | 0.02 ± 0.02 | ND | ND |
| C. jejuni 11168 (control) | 0.81 ± 0.09 | 0.05 ± 0.01 | 13.76 ± 0.34 | 0.20 ± 0.02 | 33.13 ± 0.47 | 8.09 ± 0.05 | 0.10 ± 0.00 | 0.33 ± 0.03 | 2.55 ± 0.02 | 0.10 ± 0.02 | 0.55 ± 0.16 | 26.22 ± 1.46 | 0.17 ± 0.02 | 10.21 ± 0.65 | 0.01 ± 0.01 | ND | ND |
| C. jejuni 11168 (chill) | 0.74 ± 0.00** | 0.02 ± 0.03 | 13.09 ± 0.16 | 0.21 ± 0.02 | 32.96 ± 0.14 | 8.08 ± 0.35 | 0.10 ± 0.00 | 0.27 ± 0.09 | 2.92 ± 0.27 | 0.20 ± 0.01*** | 0.66 ± 0.04 | 27.75 ± 3.05 | 0.21 ± 0.01* | 8.86 ± 2.38 | 0.05 ± 0.00*** | ND | ND |
| E. coli K-12 (control) | 3.87 ± 0.12 | 0.59 ± 0.03 | 8.62 ± 0.15 | 4.40 ± 0.16 | 31.05 ± 0.69 | 15.42 ± 0.16 | 1.21 ± 0.04 | 14.90 ± 0.22 | 0.57 ± 0.07 | 0.246 ± 0.00 | 0.20 ± 0.03 | 10.37 ± 0.28 | 0.01 ± 0.01 | 1.84 ± 0.03 | 0.05 ± 0.02 | 0.02 ± 0.00 | 0.04 ± 0.03 |
| E. coli K-12 (chill) | 3.62 ± 0.06** | 0.48 ± 0.01*** | 8.09 ± 0.25** | 3.75 ± 0.06*** | 28.81 ± 0.51*** | 16.62 ± 0.21*** | 1.06 ± 0.03*** | 14.58 ± 0.41 | 0.55 ± 0.02 | 0.24 ± 0.02 | 0.23 ± 0.06 | 13.16 ± 0.53*** | 0.01 ± 0.01 | 1.72 ± 0.12 | 0.05 ± 0.01 | 0.02 ± 0.00 | 0.04 ± 0.00 |
a
Fatty acids making up less than 1% of the total and which showed no significant difference between conditions were 18:2n-6, anteiso15:0, iso15:0, iso17:0, and CLA9c11t. Percentages are averages for three biological replicates. P values of <0.05 were considered significant. *, P values of 0.01 to 0.05; **, P values of 0.001 to 0.01; ***, P < 0.001. ND, not determined.