TABLE 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| LY180 | ΔfrdBC::(frgZmcelYEc) ΔldhA::(frgZmcasABKo) adhE::(frgZmestZPpFRT) ΔackA::FRT rrlE::(pdc adhA adhB FRT) ΔmgsA::FRT | 23 |
| EMFR9 | LY180 improved for furfural tolerance | 23 |
| TOP10F′ | F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen (Carlsbad, CA) |
| Plasmids | ||
| pTrc99a | Ptrc bla oriR rrnB lacIq | 2 |
| pLOI4315 | sthA gene in pTrc99a | This study |
| pLOI4316 | pntAB gene in pTrc99a | This study |
| Primers | ||
| pntAB cloning | Forward: CTCTCTAAGCTTGCTTGTGTGGCTCCTGACAC | This study |
| Reverse: CTCTCTAAGCTTGTTCAGTCCTCGCGGCAATC | ||
| sthA cloning | Forward: CTCTCTAAGCTTATGTTACCATTCTGTTGCTT | This study |
| Reverse: CTCTCTAAGCTTGATGCTGGAAGATGGTCACT |
a
Zm, Zymomonas mobilis; Ec, Erwinia chrysanthemi; Ko, Klebsiella oxytoca; Pp, Pseudomonas putida; FRT, FLP recombination target.