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All HCMV miRNAs are expressed in clinical isolates. Total RNA was prepared from HFF infected with two low-passage-number clinical isolates recovered from the amniotic fluid (AF2380) and urine (U8795) of congenitally infected infants. The HCMV miRNAs were detected by real-time PCR analysis. MiR-16 was used as an internal standard.
