FIG. 5.

Intracellular trafficking of labeled Tf (red) in CRFK cells containing GFP-Rab proteins (green) labeling different endosomal compartments. Colocalization is shown with Rab5 at 20 min (A) and Rab11 at 10 min (B) but not with Rab (27 min shown) (C). The large arrow highlights the perinuclear area, smaller vesicles showing colocalization are marked with small white arrows, and the cell body (white line) and nucleus (yellow line) are outlined. Retention and/or recycling of capsids or Tf to the cell surface was measured in CRFK (D) or Cf2Th (E) cells by the change in the level of fluorescently labeled ligand over time. (*, P < 0.05).