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. 2009 Aug 5;83(20):10504–10514. doi: 10.1128/JVI.00295-09

FIG. 7.

FIG. 7.

Association of CPV-2 particles with Rab11-GFP or Rab7-GFP vesicles in cells after uptake. (A and B) Time-lapse frames of CPV-2 in feline CRFK cells expressing Rab11-GFP 11 min after uptake (A) and Rab7-GFP 17 min after uptake (B). Colocalization and comovement of some particles with labeled vesicles are highlighted as individual tracks. (C and D) Colocalization of virus with Rab11-GFP after uptake into CRFK cells for 55 min (C) or Cf2th cells for 46 min (D). The white arrows highlight polar accumulations of Rab11-positive vesicles. (E and F) Colocalization of virus with Rab7-GFP after uptake into CRFK cells at 96 min or Cf2th cells at 61 min. (G and H) Examining for nuclear localization of CPV capsids that entered CRFK or Cf2th cells expressing lamin-A/C-GFP. Confocal analysis shows cells and a section through the nucleus (at the level of the blue line), as well as two Z-sections (along the red and green lines) in each case. t, time.