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. 2009 Aug 12;83(20):10644–10652. doi: 10.1128/JVI.01017-09

FIG. 2.

FIG. 2.

The C→A MHV-68 produces a full-length ORF64 protein and lacks deubiquitinating activity. (A) Lysates from uninfected (Uninf.) or WT, C→A, or (C→A)REV virus-infected radioactive [35S]methionine/cysteine-labeled 3T12 cells were immunoprecipitated with antibodies against ORF64 and gp150 followed by SDS-PAGE (8%). (B) Lysates from uninfected or WT, C→A, or (C→A)REV virus-infected radioactive [35S]methionine/cysteine-labeled 3T12 cells were treated with HA-UbVME, followed by immunoprecipitation with antibodies against ORF64, followed by SDS-PAGE (8%). (C) Lysates from uninfected or WT-, C→A-, or (C→A)REV-infected 3T12 cells were treated with HA-UbVME followed by immunoprecipitation with antibodies against ORF64 followed by SDS-PAGE (8%) and immunoblotting with anti-ORF64 antibody. (D) Lysates from uninfected or WT, C→A, or (C→A)REV virus-infected 3T12 cells were treated with HA-UbVME, followed by immunoprecipitation with antibodies against ORF64, followed by SDS-PAGE (8%) and immunoblotting with an anti-HA antibody.