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. 2009 Aug 12;83(20):10644–10652. doi: 10.1128/JVI.01017-09

FIG. 6.

FIG. 6.

The establishment of latency and reactivation of MHV-68 in the spleen are not dependent on ORF64 USP activity. (A) Real-time PCR was performed on spleens from C→A and (C→A)REV (REV) MHV-68-infected mice at 16 and 42 dpi. The data are presented as viral genome copy number relative to the copy number of rpl8. Each time point represents samples from at least five animals. (B and C) An ex vivo limiting dilution reactivation assay was used to detect reactivation from latency in cells from the spleen at 16 dpi (B) and 42 dpi (C) from C→A and (C→A)REV virus-infected mice. The presence of preformed infectious virus, as measured by using disrupted cells, is shown as open symbols. CPE, cytopathic effect. The data represent the mean value from 10 mice per experimental group ± standard deviation.