FIG. 2.

Replication of HIV-1 group M and group O viruses in single-donor PBMCs, a PBMC donor pool, and a culture of human tonsil tissue ex vivo. PBMCs from a single donor (A), a pool of PBMCs from four different donors (B), and an HLAC from a routine therapeutic tonsillectomy (C) were infected in triplicates with identical amounts of prototypic HIV-1 M strains (X4: NL4-3; R5: 49.5), HIV-1 group O primary isolates (X4: 2901; R5: 1639, 2549, 6778, 8913, 9435, 13127, and 13740), or HIV-1 group M primary isolates (X4: 2005, 2044, 2044, and ELI; R5: IN22, RU570, RW9, and D117) (each with 5 ng of p24 per well). Viral replication was monitored by assessing the concentration of p24 in the culture medium between successive medium changes on days 1, 4, 7, and 11 p.i. and is depicted for six selected viruses (left panels). Given are the arithmetic means ± SDs from one of three to four different donors analyzed for each primary cell system. The corresponding cumulative p24 production over the culture period was determined for each virus as the AUC of the mean replication kinetic (left panels), in principle as reported previously (6, 26), and is shown in the panels on the right.