TABLE 2.
Effect of the C-terminal mutations of the N protein on interactions in yeast
| pDBLeu-encoded protein (DNA BD)a | pPC86-encoded protein (AD) interactionb
|
||
|---|---|---|---|
| Empty | M | P | |
| Empty | − | − | − |
| wt N [LLD] | − | + | +++ |
| NΔ1 [LL-] | − | + | +++ |
| NΔ2 [L-] | − | − | +++ |
| NΔ3 [—] | − | − | +++ |
| N-ALD | − | − | +++ |
| N-LAD | − | − | +++ |
| N-LLS | − | + | +++ |
| N-LLE | − | + | +++ |
a
Substituted nucleotides are underlined. The carboxyl-terminal deletions of the N mutant proteins are indicated by dashes.
b
+, weak interaction; +++, strong interaction; −, no interaction.