FIG. 2.

Requirements of the PP2.1 and PP2.2 motifs for RNA replication of the FK5.1 culture-adapted replicon. (a) Colony formation assay. T7 transcripts (2 μg) were electroporated into Huh7 cells as previously described (19), 10³ transfected cells were seeded into six-well plates and selected with G418 (1 mg/ml) from 24 hpt. Colony numbers were counted 14 days later and are illustrated graphically relative to the FK5.1 replicon. *PA2.2 data were taken from (19). (b) Luciferase replicon. Huh7.5 cells were electroporated with replicon RNA and harvested into PLB at 4 and 72 hpt. Luciferase activity was measured in a luminometer as previously described (17). The data from three independent experiments are shown.