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. 2009 Aug 5;83(20):10788–10796. doi: 10.1128/JVI.02406-08

FIG. 4.

FIG. 4.

Requirements of proline 346/342 in the context of alternative genotype 1b or 2a subgenomic replicons. (a) Genotype 1b replicons. Huh7.5 cells were electroporated with the indicated replicon RNAs and harvested into PLB at 4 and 72 hpt. The luciferase activity was measured in a luminometer as previously described (17). (b) Genotype 2a (JFH-1) replicon. Huh7 cells were electroporated with the indicated replicon RNAs and harvested at 4, 24, 48, and 72 hpt. Luciferase activity was measured as described above. The data from three independent experiments are shown. Values are shown as the fold increase luciferase activity over the 4-hpt time point. (c) The 72-hpt samples from panel b were analyzed by Western blotting with antibodies to NS5A (17) or GAPDH.