FIG. 5.

Requirement for proline 342 in virus release. (a) Huh7 cells were electroporated with the indicated full-length viral RNAs and virus release into the culture supernatant measured by a focus-forming assay at the indicated time points. (b) The release of virus into culture supernatant was measured at 72 hpt for the wild-type and P342 revertant viruses. (c) Genomic RNA levels in virus preparations purified by sedimentation from culture supernatants through a sucrose cushion were measured at 120 hpt by quantitative RT-PCR. The data from six independent experiments are shown. (d) Representative anti-Core Western blot from equal amounts of virus preparations purified by sedimentation from culture supernatants through a sucrose cushion. The relative levels of Core were determined by densitometric analysis of Western blots using ImageJ software.