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. 2009 Aug 5;83(20):10582–10595. doi: 10.1128/JVI.00497-09

FIG. 6.

FIG. 6.

ORF33 is required for maturation of MHV-68 virions in the cytoplasm. 293T cells were transfected with WT BAC plus pCMV-HA (A, B, and C), 33STOP plus pCMV-HA (D, E, and F), or 33STOP plus pHA-33 (G, H, I, and J). Approximately 70-nm thin sections were examined at 4 days posttransfection by transmission electron microscopy. Putative A capsid (a), B capsid (b), and C capsid (c) were found in WT BAC plus pCMV-HA, 33STOP plus pCMV-HA and 33STOP plus pHA-33-transfected cell nuclei (Nu) (A, D, G, and H). Primary enveloped virions of 33STOP (▾)were observed in the perinuclear space (E). In the cytoplasm (Cy), viral particles, including enveloped-tegumented virions (→) were observed in WT BAC plus pCMV-HA and 33STOP plus pHA-33 transfectants (B and I), but not in 33STOP plus pCMV-HA transfectant. Partially tegumented capsids (▴) were identified in 33STOP plus pCMV-HA transfectant (F). In the extracellular space, WT BAC plus pCMV-HA and 33STOP plus pHA-33 transfectants contained virions associated with cellular plasma membrane (V) (C and J), whereas no identifiable virions are found in 33STOP plus pCMV-HA transfectant.