TABLE 1.

Primers used in this study

Primer	Sequence (5′ to 3′)	Positiona
33-01	CAGGTACCGCAACCAAAGAAGATATAAT	49592
33-02	ATCTCGAGTCAACAAGAATGTAAGTGTG	50553
33-03	TAGGAATTCGGATGGCAACCAAAGAAGATATAA	49589
33-04	TGGCTTAAGTACCATGGACTACAAAGACGA
33-05	TCACTCGAGGAGGAGGAGGATCCGGAGGAGGAGGATCCATGGCAACCAAAGAAGATAT	49589
33-06	TATGGTACCCGCCACATCACCACTTTGTAC
33-07	GTGCATGCATCCACGCTGTTCAAAATAGATT	49109
33-08	GCAGGATCCTCAATTAATCAATGTAAAGACCTTCTTTCTGC	49664
33-09	TACATTGATTAATTGAGGATCCTGCACCACGTCCATTTCA	49685
33-10	GGTGTGCATGCTACCCATCTCCAGTAGCATCAG	50244
33-11	GTGCATGCATACCTCAGTCCCACCCGTCA	48670
33-12	GGTGTGCTAGCAAGAACATCGCCCACCAGC	51609
65-01	GTCAGGGCCCAGTCCGTA	94119
65-02	TGGCCCTCTACCTTCTGTTGA	94165

^a5′ nucleotide position on the MHV-68 genome (U97553.2). Boldfacing indicates stop codons, and italics indicate restriction enzyme sites.