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. 2009 Aug 5;83(20):10770–10777. doi: 10.1128/JVI.01030-09

FIG. 4.

FIG. 4.

Effect of transient overexpression of Mcl-1 in M1-D cells infected with BeAn virus 16 h after transfection. (A) Overexpression of Mcl-1 in uninfected M1-D cells compared to nontransfected, uninfected M1-D cells, the latter lane requiring an ∼50-fold longer exposure. The Mcl-1-overexpressing cells showed a 40- to 42-kDa doublet (7) and a 34-kDa truncated form. (B) Immunoblot showing a reduced cleavage of caspases-9 and -3 to their active forms in Mcl-1-transfected versus EGFP-transfected cells infected at MOIs 10 and 20. (C) Densitometric analysis of the immunoblot data in panel B. Relative cleavage of caspase-9 and -3 to their active forms was based on normalizing the percent cleavage (cleaved product/proform plus cleaved product) in the EGFP-transfectants (MOI = 10 data). (D and E) Protection of M1-D cells from apoptosis at 8 h (P < 0.01) and 10 h (P = 0.02) p.i. by Mcl-1 overexpression (D) but not by Bcl-2 (P < 0.05) (E).