FIG. 6.

Role of Rab5 and Rab7 in BDV cell entry. Ol cells expressing the GFP-tagged versions of WT and DN Rab5 and Rab7 (Rab5 S34N and Rab7 T22N) were infected with BDV (MOI = 0.1) and at 48 h p.i. fixed and analyzed by IF using an antibody to BDV N. (A) Representative fields of cells transfected with each construct and infected with BDV. (B) Normalized numbers of infected cells. GFP-positive and GFP- and virus antigen-positive cells were counted, and normalized numbers of infected cells were obtained for each virus system by considering the number of GFP- and virus antigen-positive cells/total number of GFP-positive cells 100%. * indicates P values of ≤0.05 by Student's t test.