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. 2009 Aug 5;83(20):10480–10493. doi: 10.1128/JVI.01195-09

FIG. 7.

FIG. 7.

The H-84▿41x variant harboring the 41-residue membrane-distal stalk insertion supports virus infection and growth. (A) Microphotographs (overlays of phase contrast and fluorescence) of Vero-SLAM cells infected with recombinant recMeV-eGFP-(H-118▿41x) that contains the H-118▿41x variant instead of normal H or unmodified recMeV-eGFP for comparison. Cells were infected at an MOI of 0.01 and photographed 36 and 60 h postinfection (pI) at a magnification of ×200. Representative fields of view are shown. (B) recMeV-eGFP-(H-118▿41x) growth curves show an extended plateau phase of peak titers of progeny virus. Vero-SLAM cells were infected with recMeV-eGFP-(H-118▿41x) or recMeV-eGFP at an MOI of 0.001, and titers of cell-associated viral particles were determined at the indicated time points through TCID50 titration. Average values from three independent experiments are shown, and error bars represent SDs.