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. 2009 Sep 21;186(6):817–824. doi: 10.1083/jcb.200906168

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© 2009 Gokhale et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — CK1 is an axonemal protein located along the length of the axoneme and is extractable in 0.3 M NaCl buffers. (A) Flagella (Fla) were fractionated into a Nonidet P40 soluble membrane/matrix fraction (NP-40 Ext) and axonemal fraction (Axo), then analyzed by an immunoblot probed with the anti-CK1 antibody (top left). Axonemal CK1 was extracted with 0.3 M NaCl (top right). The bottom panels show Coomassie-stained proteins of the same samples and those used as a loading control. Positions of molecular mass standards are indicated in kD. (B) CK1 localizes along the length of the axoneme (top, “Intact Axonemes”). Biochemical depletion of CK1 removes all detectable CK1 (bottom, “Extracted Axonemes”). Bar, 5 µm.