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. 2009 Sep 21;186(6):805–816. doi: 10.1083/jcb.200903065

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© 2009 Wakabayashi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Characterization of Drp1^−/− MEFs. (A) MEFs were immunostained with anti-Tom20 antibodies. (B) Quantitation of MEFs with netlike mitochondria (n ≥ 3; >100 cells/experiment). (C) Drp1^−/− MEFs carrying pTracer-EF/Bsd (control; Invitrogen) and pTracer-EF/Bsd-Drp1 were stained with MitoTracker (Invitrogen). (D) Frames from time-lapse confocal microscopy (Videos 1 and 2). Arrowheads indicate mitochondrial division, and arrows show extension and retraction of mitochondrial tubules. (E and F) Quantitation of mitochondrial division (E) and extension (F; n ≥ 8). (G) EM of mitochondria in MEFs. (H) Peroxisomal morphology. MEFs were immunostained using anti-Pex14 antibodies. (I) EM of peroxisomes in MEFs. Peroxisomes were identified using cytochemical staining for catalase with DAB (arrows). (J) Cell proliferation (n ≥ 9). (K) Quantitation of Ki67-positive MEFs (n = 6; >100 cells/experiment). (L) Intracellular ATP levels in control (Drp1^+/+ and Drp1^+/−) and Drp1^−/− MEFs (n ≥ 6). WT, wild type; KO, knockout. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars indicate mean ± SEM.